Date of Award
© 2015 Taylor L. Follansbee
Master of Science in Biological Sciences
In the United States alone over 100 million people suffer from chronic pain and unfortunately, even still, there is a lack in scientific understanding for the mechanisms of abnormal pain sensitivity. The present study utilized a candidate gene approach to identify novel components required for modulation of the tissue damage induced pain sensitization pathway in Drosophila melanogaster. We have shown that RNAi silencing of decapentaplegic (dpp), a member of the Bone Morphogenetic Protein (BMP) signaling pathway, specifically in the class IV multidendritic nociceptor neurons significantly attenuated UV-induced nociceptive sensitization. Furthermore, overexpression of dpp in nociceptor neurons was sufficient to induce sensitization in the absence of tissue damage. We then show that the dpp receptors are required on the nociceptor neuron in order to produce allodynia, demonstrating that dpp is signaling to the very neuron that produced it. Lastly, we show that this BMP pathway is utilizing the canonical signaling SMAD factors to induce allodynia. We show that the effects of BMP signaling were largely specific to the sensitization pathway and not to normal nociception or dendritic morphology. Thus, we have shown that dpp plays a crucial and novel role in sensitization. Because the BMP family is so strongly conserved between vertebrates and invertebrates it seems likely that the genes we have analyzed represent potential therapeutic targets applicable to humans.
Follansbee, Taylor L., "Canonical BMP Signaling Is Required For Allodynia In Drosophila Melanogaster" (2015). All Theses And Dissertations. 137.