Document Type
Article
Publication Date
5-13-2014
Abstract
Epichromatin, the surface of chromatin facing the nuclear envelope in an interphase nucleus, reveals a “rim” staining pattern with specific mouse monoclonal antibodies against histone H2A/H2B/DNA and phosphatidylserine epitopes. Employing a modified ChIP-Seq procedure on undifferentiated and differentiated human leukemic (HL-60/S4) cells, >95% of assembled epichromatin regions overlapped with Alu retrotransposons. They also exhibited enrichment of the AluS subfamily and of Alu oligomers. Furthermore, mapping epichromatin regions to the human chromosomes revealed highly similar localization patterns in the various cell states and with the different antibodies. Comparisons with available epigenetic databases suggested that epichromatin is neither “classical” heterochromatin nor highly expressing genes, implying another function at the surface of interphase chromatin. A modified chromatin immunoprecipitation procedure (xxChIP) was developed because the studied antibodies react generally with mononucleosomes and lysed chromatin. A second fixation is necessary to securely attach the antibodies to the epichromatin epitopes of the intact nucleus.
Recommended Citation
Olins, Ada L.; Ishaque, Naveed; Chotewutmontri, Sasithorn; Langowski, Jörg; and Olins, Donald E., "Retrotransposon Alu Is Enriched In The Epichromatin Of HL-60 Cells" (2014). Pharmaceutical Sciences Faculty Publications. 12.
https://dune.une.edu/pharmsci_facpubs/12
Supplemental materials .zip file.
Comments
Article originally published by Taylor & Francis Group in Nucleus on 13/05/14, available online: http://www.tandfonline.com/10.4161/nucl.29141
Copyright © 2014 Landes Bioscience
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