Date of Award

Spring 4-25-2008

Rights

© 2008 Rebecca Wood

Document Type

Thesis

Department

Biological Science

First Advisor

Mark A. Sweezy

Second Advisor

Geoffrey Ganter

Third Advisor

Debra McDonough

Abstract

RecQ and Topoisomerase III proteins are both essential for proper chromosomal maintenance in species ranging from bacteria to humans. Due to their highly conserved nature throughout evolution, understanding their basic biochemical properties as well as their interactions together is important for understanding human health, disease, and even to provide potential evolutionary insight. S. pombe has been chosen as a model organism because little research has been done on the biochemical analysis of its RecQ homolog, Rqhl. This study shows developments in the biochemical characterization of a truncation protein of Rqh1 including AFM (Atomic Force Microscopy) Imaging, DNA binding, and ATP hydrolysis activity. New insight of the biochemical properties of Rqh1 will advance the development of a molecular model for Bloom's syndrome and provide a better understanding of the molecular aspects of other diseases associated with mutations m RecQ homologs.

Comments

Honors Thesis.

This digital object has been funded in part with Federal funds from the National Library of Medicine, National Institutes of Health, Department of Health and Human Services, under Contract No. #HHS-N-276-2011-0001C with the University of Massachusetts Medical School.

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