Date of Award
© 2008 Rebecca Wood
Mark A. Sweezy
RecQ and Topoisomerase III proteins are both essential for proper chromosomal maintenance in species ranging from bacteria to humans. Due to their highly conserved nature throughout evolution, understanding their basic biochemical properties as well as their interactions together is important for understanding human health, disease, and even to provide potential evolutionary insight. S. pombe has been chosen as a model organism because little research has been done on the biochemical analysis of its RecQ homolog, Rqhl. This study shows developments in the biochemical characterization of a truncation protein of Rqh1 including AFM (Atomic Force Microscopy) Imaging, DNA binding, and ATP hydrolysis activity. New insight of the biochemical properties of Rqh1 will advance the development of a molecular model for Bloom's syndrome and provide a better understanding of the molecular aspects of other diseases associated with mutations m RecQ homologs.
Wood, Rebecca, "Cloning And Biochemical Characterization Of The RecQ Helicase And Topoisomerase III In Schizosaccharomyces Pombe" (2008). All Theses And Dissertations. 6.